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27072-45-3 异硫氰酸荧光素

异硫氰酸荧光素

英文名称:Fluorescein isothiocyanate
CAS:

27072-45-3

分子式:C21H11NO5S
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  • 化合物简介
  • 基本信息
  • 编号系统
  • 物化性质
  • 安全信息
  • MSDS
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异硫氰酸荧光素简介

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.
A succinimidyl-ester functional group attached to the fluorescein core, creating "NHS-fluorescein", forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles.
FITC has excitation and emission spectrum peak wavelengths of approximately 495nm/519nm. Like most fluorochromes, it is prone to photobleaching. Because of the problem with photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed.

异硫氰酸荧光素基本信息

中文名称 异硫氰酸荧光素 英文名称 Fluorescein isothiocyanate
中文别名

荧光素5(6)-异硫氰酸酯; 异硫氰酸荧光素(5-,6-异构体混合物); 异硫氰荧光素; 异硫氰酸荧光素(FITC);

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英文别名

5-FITC; Isothiocyanatofluorescein; 5-Isothionatofluorescein; FITC isomer I; Fluorescein Isothiosyanate; fluorescein isothiocyanate isomer 1; FITC ISOMER; FITC-I; Fluorescein 5(6)-isothiocyanate; FITC; Fluorescein-5-isothiocyanate; Fluorescein Isothiocyanate;

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CAS号 27072-45-3 分子式 C21H11NO5S
分子量 389.38100 精确质量 389.03600
PSA 120.44000 LOGP 4.40010

异硫氰酸荧光素编号系统

UNII 7BUU93N0HM

异硫氰酸荧光素物化性质

密度:
1.54g/cm3
沸点:
708.6ºC at 760mmHg
熔点:
>360ºC(lit.)
闪点:
382.4ºC
折射率:
1.753
储存条件:
2-8ºC
蒸汽压:
9.53E-21mmHg at 25°C

安全信息

安全说明:
S22
危险类别码:
R42/43
WGK Germany:
3
危险品标志:
Xn
信号词:
Danger
危险性防范说明:
P261; P342 + P311
危险标志:
GHS08
危险性描述:
H334
SDS 1.0 中文 展开
SDS 1.0 英文 展开
MSDS 中文 展开
1H NMR : Predict展开

核磁图谱 1H NMR : Predict

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异硫氰酸荧光素
CAS号:27072-45-3 分子式:C21H11NO5S 分子量:389.38100

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